MT, catalytically inactive mutant.
ITC can characterize protein fragments and catalytically inactive mutant enzymes.
Cells expressing the active ezrin mutant (T567D) showed significantly less preservation injury compared to wild type or the inactive mutant (T567A), while ezrin-specific siRNA knockdown and the inactive mutant potentiated preservation injury.
Stable Chinese hamster ovary (CHO) cell lines expressing either human FAAH or an inactive mutant, FAAH-S241A, were established.
This effect of HDAC6 was abrogated by overexpression of a catalytically inactive mutant of HDAC6, which harbors H216A and H611A mutations in the deacetylase domains (Fig. 4A D).
The "rescue" function of Rubisco was similarly demonstrated by constructing its inactive mutant RbcL197 and comparing its performance with the wildtype enzyme.
For this purpose, we first infected BV2 cells and primary microglia with lentivirally expressed SIRT1-WT as well as its catalytically inactive mutant SIRT1-HY.
In order to understand the role of the redox chemistry in their bifunctional activity, catalatically-active and inactive mutant proteins have been probed in spectroelectrochemical experiments.
A catalytic inactive mutant Ser181Ala of B. pumilus AXE was also engineered, expressed, purified and crystallized for functional and structural studies.
This is a biologically inactive mutant we found incorporating into exosomes at high levels also when fused at its C-terminus with foreign proteins.
As controls, we used both the wild-type enzyme and the catalytically inactive mutant C178S.
