Sentence examples for cold mutant from inspiring English sources
The localisation of Nrg-GFP was perturbed in all epithelial tissues examined in cold mutant embryos (Fig. 3A H).
For instance, it has been shown that the SJ component NrxIV is re-localised to internal vesicles in cold mutant embryos [10].
To test whether this feature also applies to the cold phenotypes, we directed expression of a FLAG tagged form of Cold in the tracheal cells of a cold mutant embryo, using the btlGAL4 driver.
In fact, the SJ markers Nrg-GFP and NrxIV were not seen accumulating at the contact regions between these cells in a cold mutant background (Fig. 5D–E' and H–I').
Indeed, in mosaic third larval instar wing discs containing large coldf02290 Minute+ clones, we observed that the cold mutant cells fail to accumulate the FasIII marker in their most apical part, unlike the surrounding wild type cells (Fig. 7A,A').
Our results show that a 10 kDa dextran dye readily diffused into the ventral cord of cold mutant embryos, whereas it was efficiently excluded from this structure in wild type embryos of the same age (Fig. 5A B).
We show that cold mutants display similar phenotypes to those seen in bou alleles [7], indicating that cold is essential for the organisation of the insect septate junctions (SJ).
Given that cold is expressed in surface glial cells, we monitored if dye injected into the hemolymph could penetrate into the ventral cord of cold mutants 22 hours old, when the blood-brain barrier is fully established [26].
Our genetic characterisation of cold mutants further confirms that this gene is required for SJ organisation in epithelial tissues, and shows by direct comparison with bou mutants that both elicit undistinguishable phenotypes.
Staining of the embryonic tracheal system with the 2A12 luminal marker revealed that the overall organisation and branching pattern of this tubular network was preserved in cold mutants (Fig. 2C D).
Analysis of cold mutants shows that this gene is specifically required for the organisation of the septate junctions in epithelial tissues and in the nervous system, where its contribution is essential for the maintenance of the blood-brain barrier.
