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ES cells containing this "silent" mutant allele are thus heterozygous at the RIα locus.
The silent mutant Cyr61 was delivered into the Cyr61-silenced Du145 cells with lentivirus.
Flag-tagged silent mutant LAMR was detected using the Flag M-2 antibody from Stratagene.
A silent mutant Cyr61 expression construct was made resistant to an siRNA sequence.
Silent mutant LAMR cell lines were generated by transfecting HT1080 cells with pCDNA 3.1/c N-terminal flag vectors containing either full-length or 1 220 C-terminal-truncated silent mutant LAMR.
Consistent with this, the introduction of the silent mutant Cyr61 rescued Cyr61 expression and the proliferation of Du145 cells.
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Emphasizing the importance of vigorous blood circulation on HSC formation, the zebrafish silent heart mutant that lacks a heartbeat and bloodflow, had reduced HSC formation.
NO production increases in response to sheer stress and blood flow [ 69], and remarkably, SNAP treatment of silent heart mutant zebrafish embryos could rescue HSC development.
Upon sequencing the mutant alleles, we found that both pro3 -1 and pro3 -2 mutant alleles each have four missense mutations and one silent mutation in their open-reading frames.
One strain (STM043/05) consisted of a silent mutation in the QRDR and an additional three mutations compared to other mutants.
A SNP in which both the original codon and the mutant codon produce the same protein sequence is called a synonymous polymorphism or silent mutation.
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