Sentence examples for buffer samples were boiled from inspiring English sources

Samples were then incubated for 2(h at room temperature followed by extensive washing. After adding SDS-PAGE sample buffer, samples were boiled and centrifuged (10(min, 16 000(g), and the supernatant subjected to SDS-PAGE.

Following the addition of sample buffer, samples were boiled for 3 min and separated by SDS-PAGE (8% acrylamide).

After addition of Laemmli buffer, samples were boiled for 5 min at 96 °C and separated by SDS-polyacrylamide gel electrophoresis.

Following protein determination (n=3, Bradford, 1976) and addition of sample buffer, samples were boiled at 95°C for 5 min and solubilised proteins (50 μg) were separated by sodium dodecyl sulphate (SDS) polyacrylamide gel (10%) electrophoresis.

Immune complexes were collected with protein G agarose beads (Pierce Biotechnology, Rockford, IL) followed by several washes in lysis buffer, samples were boiled and then subjected to SDS-PAGE/Western blot.

After washing with buffer A samples were boiled in 1× laemmli buffer for 10 min and analyzed by Western blotting using 3% skim milk in TBST for blocking, anti-flag antibody 1 1000 (Sigma Aldrich, F1804) and polyclonal anti-myc antibody 1 1000 (Santa Cruz, SC-789) as primary antibodies.

After three washes with NP-40 lysis buffer, the samples were boiled with 2× SDS loading buffer and subjected to SDS-polyacrylamide gel electrophoresis (PAGE) and Western blotting analysis.

After adding of Laemmli buffer [63], samples were boiled for 5 min and 60 mM final concentration DTT, was added.

After adding loading buffer, the samples were boiled for 10 min and resolved by Tris/Glycine SDS-Polyacrylamide gel electrophoresis, and the proteins were then transferred to a nitrocellulose membrane (Amersham) in the presence of 20% methanol and 0.1% SDS.

After adding sample buffer, the samples were boiled for 5 min at 95°C.

After supplementation of 25 µl of protein sample loading buffer the samples were boiled for 5 min.