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The protein as well as the buffer samples were injected automatically using the sample-changing robot for solution scattering experiments at the SAXS station X33 [ 27].
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Buffers and samples were injected by a nonpulsatile piston pump into the 30 μL flow cell, which was mounted on the coupling prim.
Samples were injected and eluted with 1.2 column volumes of running buffer at a flow rate of 1 mL/min.
Samples were injected in Nanopure H2O and eluted at 0.02 mL/min in phosphate buffer (50 mM sodium phosphate, 150 mM NaCl, pH 7.0).
The samples were injected onto the analytical column (Partisil 10 ODS) eluted with acetonitrile/0.01 M acetate buffer (pH 5.3) at a flow-rate of 2 ml/min and detected at 240 nm.
In brief, the lipoprotein samples were injected through a UnoQ12 column (BioRad, Hercules, CA) that had been equilibrated with buffer A (0.02 mol/L Tris-HCl, pH 8.0, containing 1 mmol/L EDTA).
Samples were injected directly into the cuvette and excess sample was removed by repeatedly diluting the cuvette content in buffer.
Protein samples were injected onto a Tosoh GC-PAK 200 gel filtration column that was pre-equilibrated with buffers in the presence or absence of Ca2+.
Samples were injected in a splitless mode.
10 μl samples were injected into the HPLC apparatus.
Samples were injected in split-less mode at 280°C.
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