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Following addition of 400 µl of 1 X Binding Buffer, samples were processed by flow cytometry.
After three washes with potassium phosphate buffer, samples were processed for embedding into London Resin White (LRW) (EMS) or paraffin (PolyFin, Polyscience).
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The processing phase, in which buffered samples are processed for spectrum analysis.
Spectrum sensing generally consists of two phases: The sampling phase, in which raw samples are collected from the air The processing phase, in which buffered samples are processed for spectrum analysis.
Following washing in NP-40 lysis buffer, the samples were processed for Western blotting.
Following further washing for 24 h in 10% buffered formal saline, tissue samples were processed and embedded in paraffin wax.
To exclude artifacts resulting from small differences in buffers and incubation times, all samples were processed simultaneously, and images were recorded using identical settings on the microscope and the software.
Protein lysates were centrifuged to pellet cellular debris, and the supernatant was removed and quantified using DC Protein Assay (Bio-Rad Inc .. Protein samples were processed in urea buffer and subjected to 2D-DIGE analysis (Applied Biomics , Inc Hayward, CA).
Bone marrow samples were processed with a similar protocol, but using double volumes of buffer AL and ethanol.
Samples were processed for western blotting as described previously using NP40 lysis buffer at at 4°C [ 8, 9].
Samples were processed according to manufacturer's instructions and eluted in 60 μL elution buffer.
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