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After two washing steps in the same buffer, samples were postfixed with 1% (w/v) OsO4 in cacodylate buffer for 2 h at 4°C, washed again, dehydrated and embedded in an Epon-Araldite mixture according to standard protocols.
Following six transfers in 0.1 M sodium cacodylate buffer, samples were postfixed in 1% osmium tetroxide in the same buffer for 1 h in the dark, washed in distilled water, and stained in 1% aqueous uranyl acetate for 1 h in the dark.
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After the cells were rinsed with phosphate buffer, the samples were postfixed in 1% osmium tetroxide for 1 h, rinsed with water, dehydrated in a graded ethanol series, incubated with propylene oxide, and kept overnight in Epon812.
After washing in buffer, the tissue samples were postfixed in 1% osmium tetroxide for 2 h at 4°C.
The samples were postfixed in buffered osmium tetroxide, dehydrated in ascending grades of ethanol and embedded in Epon.
After several washes with 0.15 M sodium cacodylate the samples were postfixed in the same buffer but additionally containing 1% osmium tetroxide (Science Services, München, Germany).
After three other washes with cacodylate buffer at 4°C for 10 min each, the samples were postfixed for 15 min at room temperature with 2% osmium tetroxide in deionized water.
Samples were postfixed in 1% phosphate buffered osmium tetroxide, dehydrated through a graded acetone series, and embedded in an Araldite 502/Epon 812 resin.
Then samples were postfixed with 1% OsO4 in phosphate buffer (pH 7.0) for 1 hour and washed three times with the same phosphate buffer for 15 min.
After being washed with PBS for 3 times, the samples were postfixed in 1% osmium tetroxide in cacodylate buffer (pH 7.2) for 1 hour.
Samples were postfixed with osmium tetroxide (2%) in sodium cacodylate buffer, dehydrated and embedded in epoxy resin.
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