Sentence examples for buffer samples were supplemented from inspiring English sources

Matrix and buffer samples were supplemented with 13 internal standards for retention index correction (C8-C30 facid acid methyl esters) as previously reported [31].

After sonication, the samples were supplemented with 480 μl of RIPA ChIP buffer (RIPA buffer including 20 mM butyrate and protease inhibitor) and centrifuged at 12,000 ×  g for 10 min at 4°C.

Samples were supplemented with SDS-loading buffer, heated (5 min, 65 °C) and loaded on a 12% SDS-polyacrylamide gel, followed by immunodetection against HA-tagged Pun1p.

All the samples were supplemented with 4 μl 4x Laemmli sample buffer (Bio-Rad Laboratories, HerCAles, CA, USA), filled up with distilled water to a final volume of 16 μl, and incubated for 10 min at 95°C for denaturation.

The samples were supplemented with 6.25 μL of 5× gel loading buffer and loaded into a native 2% agarose gel.

Ten to twenty microgram sample were supplemented with 5× Laemmli buffer and boiled for 5 min at 96°C.

Lysis and wash buffer were supplemented with 5 mM imidazole and elution buffer supplemented with 300 mM imidazole.

H-Ras purification buffers were supplemented with 2 mM MgCl2.

Wash buffers were supplemented with 0.1% SDS.

All buffers were supplemented with protease and phosphatase inhibitors (Sigma).

Samples from cell extractions (typically containing 30 to 100 μg of protein) were resolved by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) on 10% polyacrylamide gels (29 1 acrylamide:bisacrylamide ratio) under reducing conditions (with the sample buffer being supplemented with DTT to 100 mM) before transfer by electroblotting to PVDF membranes (Immobilon P, Millipore).