Sentence examples for buffer samples were incubated from inspiring English sources

After three washes and 5 min blocking in blocking buffer, samples were incubated with a polyclonal anti-Neisseria antibody (diluted 1∶100 in blocking buffer) for 1 hour, to detect intracellular and extracellular bacteria.

Instead of incubating and rotating samples at room temperature after the addition of CTAB buffer, samples were incubated at 55°C without rotation.

Upon adding SDS-loading buffer, samples were incubated at 95°C and analyzed by SDS-PAGE and immunoblotting using anti-σK antibodies (Resnekov et al., 1996 ).

After being washed in 0.1 M cacodylate buffer samples were incubated for 2 h in 1% osmium tetroxide solution, dehydrated with an increasing series of ethanol and xylene, and were allowed to polymerize in Epon (Serva).

Following washes in blocking buffer, samples were incubated with a goat anti-rabbit secondary antibody conjugated to 10 nm colloidal gold (diluted 1/200 in blocking buffer; BB International) for 45 min.

After removing blocking buffer, samples were incubated with primary antibody in blocking buffer overnight at 4°C and then washed three times with IF wash buffer, followed by incubating with Alexa Fluor 488-conjugated secondary antibody (Life technologies) for 40 minutes in a dark humidified chamber and then washed three times with IF wash buffer for 20 minutes.

After 3 washes in PBS/Tween buffer, samples are incubated for overnight at 4°C.

Plasma samples were treated for 2 h at 37 °C with 200 U of ß-glucuronidase (HP-2 type) in 0.5 ml of 0.2 M acetate buffer; control samples were incubated with buffer alone.

After addition of 80 μl of a solution containing anti-histon-biotin antibodies and anti-DNA-peroxidase antibodies in incubation buffer, the samples were incubated under moderate shaking for 2 h.

After addition of chaotropic lysis buffer, the samples were incubated for 15 min at 58°C, followed by incubation with DNA binding beads for 15 min.

Following washes with buffer A, samples were incubated with the amplification solution for 100 min at 37 °C in humidity chamber and then washed with buffer B, followed by another wash with buffer B × 0.01.