Sentence examples for buffer samples were dehydrated from inspiring English sources
After a post-fixation step in 2% osmium tetroxide in 0.1 M sodium cacodylate buffer, samples were dehydrated through a graded ethanol series followed by acetone and finally embedded in Epon resin (Sigma-Aldrich, Buchs, Switzerland).
Following an overnight rinse in 0.1 M phosphate buffer, samples were dehydrated through a graded series of ethanol solutions, then propylene oxide.
After post-fixation in 1% osmium tetraoxide (in 0.2 M cacodylate buffer), samples were dehydrated in a series of ethanol concentrations.
After post-fixation for 30 min with 1% OsO4 (EMS), 3.8% potassium ferricyanide (Vetec Química Fina, Rio de Janeiro, RJ, Brazil) and 2.5 mM CaCl2 (Sigma Aldrich) in the same buffer, samples were dehydrated in a series of acetone (30% 100%), and finally embedded in Epon 812 (EMS) for TEM.
After three washes with the buffer, samples were dehydrated through an acetone series and embedded in a mixture of EPON 812 (17%), Araldite 502 (13%), and dodecenyl succinic anhydride (67%) containing 3%DMP-30DMP-30n accelerator.
After three buffer washes, samples were dehydrated by ethanol series and embedded in Epon 812.
After post-fixation in 1% osmium tetroxide and 0.1 mol/L of phosphate buffer, the samples were dehydrated in graded series of alcohol and embedded in epoxy resin.
After a cacodylate buffer wash, samples were dehydrated through a graded series of ethanol washes, followed by critical point drying using a Bal-Tec CPD 030 (Bal-Tec AG, Liechtenstein).
After rinsing in phosphate buffer, the samples were dehydrated in a series of graded ethanol and air-dried.
Following a brief rinse in the buffer, the samples were dehydrated in an ethanol series for 1 h at each gradation.
Briefly, cells were fixed in 4% paraformaldehyde in 0.2 M Sörensen buffer; the samples were dehydrated with increasing concentrations of ethylic alcohol and infiltrated with LR-White hydrosoluble resin (London Resin Co., Hampshire, United Kingdom).
