Sentence examples for buffer samples were analyzed from inspiring English sources

After additional washing with FACS buffer samples were analyzed blinded using a BD LSRFortessa™ cell analyser (BD Biosciences, Allschwil, Switzerland).

Following three washing in staining buffer, samples were analyzed by FACS using a flow cytometer LSR II (Becton Dickinson, San Jose, CA, USA).

Supernatants and pellets were brought to equal volumes of SDS/PAGE Laemmli buffer, and samples were analyzed by SDS/PAGE using 4 12% Bis-Tris NuPAGE gel in MES buffer (Life Technologies) followed by anti-GST immunoblotting.

After boiling in 20 mL 2 × SDS sample buffer, the samples were analyzed by western blotting.

The reaction was stopped by adding Laemmli's buffer and samples were analyzed by western blotting.

After Laemmli buffer addition, samples were analyzed by sodium dodecyl sulfate (SDS-PAGE) and western blotting using specific antibodies.

After adding additional 400  μl of 1 × binding buffer, the samples were analyzed by flow cytometry within 1 h.

Bound proteins were eluted in 2x reducing sample buffer, and samples were analyzed by Western blotting as described using mouse anti-Smad4 antibody for Smad4 and rabbit anti-SUMO1 or SUMO2/3 antibody for SUMO-conjugated Smad4 protein.

The beads were then washed for three times with 1 ml of IP buffer and eluted samples were analyzed by immunoblot using anti-Myc (Cell Signaling Technology, Beverly, MA) and anti-GFP antibodies.

After 30 min 300 μl of Annexin binding buffer was added and samples were analyzed on BD LSR-II flowcytometer (BD Biosciences, Mississauga, ON, Canada).

After the incubation period, 400  μL of the 1x annexin-binding buffer was added and samples were analyzed by flow cytometry on LSRII (Becton Dickinson, San Jose, CA, USA) flow cytometer and 5 × 10 cells were analyzed per sample in three independent experiments.