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Exact(5)
The TCTP and its variants were cloned in SfiI site between the leader sequence and the rabbit Fc region.
On the k-light chain, the section GVDGDIVMTQ from G29 to Q38 is a repeat of a preceding section from G17 to Q26, and forms the junction between the leader sequence and first framework region of the mature light chain.
(A, 1) The 3′-end of an incoming protospacer attacks the chromosomal CRISPR locus at the boundary between the leader sequence and repeat 1.
To obtain a secreted, tumour-targeted form of carboxylesterase-2 the cDNA encoding the human scFv antibody C28 directed against the epithelial cell adhesion molecule EpCAM, was inserted between the leader sequence and carboxylesterase-2.
The supporting evidence includes bidirectional best hit matches between the leader sequence regions, similar Cys-rich tripeptide repeat structures in the C-terminal regions, and colocalization of the B. licheniformis member with two distinctive components, the cyclodehydratase and the scaffolding protein, of canonical heterocycle biosynthesis cassettes.
Similar(55)
Yet, not only leaders are not highly conserved but divergence is very high between the leader sequences of the putatively active CRISPR1 and CRISPR2 in all Escherichia and some Salmonella genomes.
For the CRISPR adaptation process in vivo, integration occurs at the junction between the first repeat and the leader sequence, which immediately suggests a role for sequence specificity in the reaction.
The leader sequence of the endogenous gene serves as the antisense target in the gene-switching method.
Based on sequence similarity between the three leader sequences, a common reverse primer was designed to align to the conserved region in the leader sequences of all three arrays and forward primers aligning specifically to spacers proximal to the leader sequence in each array (Additional file 9).
Mfold analysis indicates that the leader sequence forms a conserved (>90%) P0 duplex with the linker sequence between the two aptamers.
What is the role of the leader sequence?
Related(18)
between the leader peptide
between the region sequence
between the percept sequence
between the training sequence
between the testing sequence
between the microRNA sequence
between the attB1 sequence
between the polymerase sequence
between the reference sequence
between the cDNA sequence
between the genome sequence
between the base sequence
between the seed sequence
between the protein sequence
between the target sequence
between the query sequence
between the consensus sequence
between the coding sequence
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