This potential suggests that by routinely applying the present analysis to different nucleic acid sequences undergoing various structural changes, it may be possible to achieve a detailed and robust understanding of the relationship between base composition, base sequence and structural heterogeneity in nucleic acids.
One strand is held to another by hydrogen bonds between the bases; the sequencing of this bonding is specific i.e., adenine bonds only with thymine, and cytosine only with guanine.
Discrimination is based on a kernel machine binary classifier [ 50] capable of computing similarity between base sequences in terms of their respective putative secondary structures [ 34] and thus of the spatial conformation of their binding sites (see Methods for a detailed description of the classifier).
Thereby, the objective of every SVR was to learn a quantitative relationship between the sequence based features (see Table 2) and the PFM similarity of the TFs (see Section 'Low-level similarity score for PFMs').
At A, X, and C, the NOE cross-peak intensities between the base protons and the deoxyribose H1′ protons were of the same relative magnitudes as those between other bases in the sequence.
After membrane disruption, it binds to DNA by intercalating between the bases with little or no sequence preference.
Surprisingly, when the sequence between bp 1,451 and 3,513 in Intron 9 was further deleted from Δ1 to yield Δ2, a significant Exon 10-containing Band II was obtained, along with a Band I which contained a novel Exon 10a consisting of two segments of base sequences located between the E9 and E10 sequences (Figure 2B and Figure S4).
Accordingly, the angle formed between the diagonal and the base in this sequence increases continually from just over 45° to just under 60°.
Each miRNA-Ago complex interacts with a specific mRNA, typically through pairing of nucleotide bases between the miRNA sequence and complementary sequences in the mRNA's 3-untranslated region (3'UTR), which results in translation repression or degradation of the mRNA.
Single-end, 100 base sequencing generated between 7 and 12 million reads per library.
