These HSPs scores reflect the similarity between the test sequence and all training sequences in the training sets.
The SDI algorithm was used to identify the type of evolutionary relationships (orthology/paralogy) between the tested sequence and the subfamilies of genuine oxygenases, using the established evolutionary tree of species [ 28] as the reference.
It should also be noted that although a rather relaxed E-value (0.0001) cut-off was used, the observed E-values between the test sequence and the closest query sequence were much below this threshold indicating that the hits obtained were highly significant.
Identity values between the test sequences and the references were calculated using the MEGA5 program with the pairwise deletion option.
To estimate the similarities between the test sequences, the identity values were used as input characters for the UPGMA algorithm (Murtagh 1984) with root mean square deviation (Huang et al. 2005).
However, when analysed individually, we found that 4 out of 6 males and 1 out of 6 females showed significant difference between two categories of motor pattern (two sample t test: bottom row of Table 1).> -wrap-foot> In case of analysis for motor learning, the RTs were compared between the test sequences with and without motor pattern consistent to the baseline sequence.
Therefore, the sequences of the reporter mRNA fragments are independent from the test sequence inserted between the ribozymes (Figure 1A, below large arrows).
To prevent the occurrence of systematic differences between observers because of repeated testing, the test sequence, and thus observer order, was randomized.
The test sequence in this project is represented by a feature vector that consists of a list of similarity scores between test and training sequences.
If the -10 and -35 region motif score above the threshold and exhibit a spacer length between 16 20 bp, the test sequences were signed as TSS with indicated promoter.
