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NTA generally induce a divergence between the microRNA sequence and its corresponding genomic sequence.
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A microRNA-specific looped primer is used for the reverse transcription, thus extending the microRNA sequence enabling detection with Taqman® real-time PCR.
The microRNA sequencing and degradome sequencing developed by Solexa (Illumina Inc).
For the FAMAp:amiRBR construct, the artificial microRNA sequence was designed with the Web MicroRNA Designer platform (http://wmd3.weigelworld.org).org
The "Vari" filter assumes a separation relative to a maximum score for the specific microRNA sequence.
In plants they repress gene expression by catalytic mRNA degradation on the basis of sequence homology between the microRNA itself and a target sequence.
The potential read/known microRNA sequence pairs were then aligned by Needleman-Wunsch dynamic programming algorithm.
Although the results of our microRNA sequence analysis showed that the imprinted Dlk1/Gtl2 microRNAs are well conserved between mouse and human, we find that the mature microRNAs are quite distinct among each other in terms of their sequences and of the genes they are predicted to regulate.
The results of our microRNA sequence analysis show that microRNAs encoded within the Dlk1/Gtl2 region are well conserved between mouse and human, both in terms of their pre-microRNA and mature sequences.
One is based on the total tag count (#specific microRNA tags/ total microRNA sequence tags) and the other is based on the most abundant tag count (#the most abundant tag of specific microRNA/ total microRNA sequence tags).
Possibly as a consequence of mature microRNA sequence divergence, the predicted microRNA target genes are diverse.
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