Sentence examples for mutant were purified from inspiring English sources

His-tagged proteins (CG9523 and its mutant) were purified using Ni2+ affinity purification (Qiagen) and were aliquoted at stored at −80°C in 20 mM Tris, pH 7.5, 5 mM NaCl, 1 mM DTT, and 10% glycerol.

The C-terminally hexahistidine-tagged form of the P. aeruginosa DinB protein [20], and the exonuclease-deficient (D219A) bacteriophage T4 DNA polymerase (T4 exo– Pol) mutant were purified and quantified as described [60], [61].

The outer dynein components from isolated axonemes of wild-type and dyf13 mutant were purified and quantified by SDS-PAGE.

Cells were harvested by centrifugation at 4000 r.p.m. for 15 min. Lig1 and Lig1 PIP mutant were purified identically.

To this end, Imp2 and the Imp2-Dimer-Dimer-4A mutant were purified from S. pombe cell lysates and assayed by analytical ultracentrifugation.

Histidine-tagged myristoylated Arf1, Arf6 and the Arf6 K3E mutant were purified from baculovirus-infected Sf9 (Spodoptera frugiperda 9) insect cells using nickel agarose beads and gel filtration.

The OsFRO1 mutant was purified and designated 'MuFRO1'.

The Annexin A2-K302A mutant was purified with the same procedure.

Each mutant was purified as described above for the wild-type enzyme.

HRSV/FTM-NN mutant was purified following procedures used previously for purification of analogous mutants from supernatants of Hep-2 cells infected with rVV [ 19].

To avoid interference from phosphorylations at these residues, an S12A/S14A mutant was purified and subjected to in vitro phosphorylation by the above kinases.