Sentence examples for mutant were prepared from inspiring English sources

The full-length HIV-1 WT IN, the 50-212 corelytic core domain (CC), the 1-212 two-domain protein (ΔC) and the E152A point mutant were prepared as previously described [21].

Probes of the sequences 5'-tttcttgggcttccttgttttgacttgtaaccataaat-3' and 5'- tttcttgggcttccttggggtgacttgtaaccataaat-3' (mutant) were prepared by end labeling annealed oligos with γ32P-ATP using T4 Polynucleotide Kinase (Promega) and purification with MicroSpin™ G-50 Sephadex columns (Amersham Biosciences).

The recombinant HA-Wss1 protein and HA-Wss1-AQA mutant were prepared similarly except that the Ni-NTA purification step was omitted.

Heat inactivated bacteria of wild-type and ΔSPI-1 mutant were prepared by heating the culture to 65 °C for 30 min.

To further demonstrate the biological effect of G and GA KRAS mutants, NIH3T3 cells which stably transfected with empty vector, KRAS-WT, KRAS-G12V, G or GA mutant were prepared.

Based on prior studies, the G11C/U27Δ double mutant was prepared.

To weaken this structure (by ΔG of about 20 kJ/mol) the same double mutant was prepared using another mutagenic oligonucleotide with silent mutations in the Ala1 (GCU) and Leu2 (UUG) codons.

The FYVE-CENT R1945Q mutant was prepared by PCR site-directed mutagenesis.

A G27 ΔHP0723::KanSacB, KanR, Sucrose Sensitive mutant was prepared using the primers of Supplementary Table S1.

Total RNA from wild type and mutant was prepared for small RNA Sequencing-by-Synthesis according to the prescribed procedure and standards of the Illumina Sample Preparation Protocol.

K359A Smutantutant was prepared by replacing the wild-type Lys359 codon (AAA) with an Ala codon (GCA) using oligonucleotide-directed mutagenesis.