Sentence examples for transformants were prepared from inspiring English sources

For plasmids to be digested with ClaI, the three plasmids were transformed into E. coli strain DM1, and transformants were prepared using the Qiagen Plasmid Maxiprep kit (Valencia, CA), according to the manufacturers instructions.

Frozen stocks of single transformants and pools of transformants were prepared by standard methods.

Whole-cell lysates of the transformants were prepared and equal amounts of protein from each strain were subjected to immunoblot analysis.

Inoculum cultures of the two S. cerevisiae transformants were prepared in 500 ml shake flasks with 140 ml of SC-Ura medium (excluding the agar).

PCR templates for genotyping the G418-resistant transformants were prepared from cells grown on the agar plates as follows: the cells were suspended in 50 μl of TE buffer (pH 8.0), disrupted by freezing in liquid nitrogen and thawing at 70 °C, and cell debris was removed by centrifugation.

Genomic DNA from the transformants was prepared using phenol chlorophorm extraction method.

The nuclear fractions of each transformant were prepared for the sequential immunoprecipitation and western blotting as described elsewhere.

To determine the fatty acid composition of the yeast transformants, fatty acid methyl esters (FAMEs) were prepared using the method modified from Lepage and Roy [ 44].

Transformants were grown out and competent cells were prepared following Inoue's protocol [ 17].

The transformants were treated with MMS and cell extracts were prepared at different time points.

Plasmids were prepared from cultures of individual ampicillin-resistant transformants.