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To understand this high TCL resistance in the G93V mutant, we obtained the crystal structures of mutated ENRs complexed with TCL and NAD+.
Each new dumpy mutant we obtained was phenotypically characterized by crossing it to dpov1 flies and to flies carrying Df 2L ED250, which deletes the entire dumpy gene.
Starting with purified genomic DNAs of strains 34F2 and gerH::ery mutant we obtained the draft sequences of each of these strains in 24 hours using the FLX protocol.
The butanol-tolerant irrE mutant we obtained has significant growth advantages in the presence of 0.125% to 0.875% butanol (see Figure 4), and was able to reach an OD value of more than 2 in the presence of 0.125% and 0.25% butanol, similar to that of the control strains in the absence of butanol.
Using the Arabidopsis ndr1-1 null mutant, we obtained genetic and molecular evidence that at least one of our candidate genes is a functional NDR1 ortholog.
For this mutant we obtained almost no binding to lipid mixtures with phosphatidylglycerol, but an enhanced interaction with liposomes containing galactosyldiacylglycerol (Fig 1, 2, 3).
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Among the RP subunit mutants we obtained, two independent alleles of rpt2a and rpn2a showed hypersensitivity to high-B stress.
More than ninety mutants we obtained in this study were distinguished by a high level production of bio-ethanol as compared to the diploid control strain.
From a screen of 2000 mutants we obtained a disruptive integration in the Tcf3 gene.
The ethanol-tolerant irrE mutants we obtained will be useful for the design of ethanol-producing strains.
After screening of the mutants, we obtained a line for TD21D12.3 (tm3004) and another line for T07A9.1 tm30455).
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