Sentence examples for mutant we expressed from inspiring English sources

In the case of the pcnaK164R mutant we expressed mRFP-pcnaK164R.

To determine the cellular localization of each mutant, we expressed each in HeLa cells and visualized localization with indirect immunofluorescence (Figure 3C).

In this mutant, we expressed aPKCΔN, a gain-of-function form of aPKC where the N-terminal domain normally used for binding to Par-6 and restricting the localization and activity of aPKC is deleted [25].

For complementation of GCP-WD-depleted cells with exogenous GCP-WD and GCP-WD mutants we expressed from a single plasmid shRNA targeting endogenous GCP-WD and RNAi-insensitive EGFP-tagged GCP-WD or GCP-WD mutants.

To investigate the substrate interactions of the domain I mutants, we expressed each HCCS mutant with (or without) the cytochrome c substrate.

To examine the impact of our GPN2 and GPN3 mutants we expressed a functional GFP-tagged version of Rpb1 in each mutant.

To test whether Fam20A could stimulate kinase activities of Fam20C mutants, we expressed Fam20A with Flag-tagged Fam20C mutants and monitored V5-tagged OPN phosphorylation by its mobility during SDS-PAGE.

To investigate the effect of the small subunits on the new HSAN1-associated hLCB2a mutants, we expressed each of the human mutant genes in yeast cells which had had the endogenous subunits of SPT knocked out.

To determine whether DAF-16 translocates to the nucleus in long-lived Calcineurin mutants, we expressed DAF-16::6xHis::GFP using a daf-16 promoter in the tax-6 ok2065) tax-6 ok2065

To further investigate the deleterious effects of EF-G1 missense mutant proteins, we expressed the II032 mutant form CG4567-G538E in various tissues.

To test whether AaTTG1 protein can rescue the Arabidopsis ttg1 mutant phenotype we expressed the Arabis alpina coding sequence under the promoter of the Arabidopsis TTG1 gene [ 49].