Sentence examples for mutant we carried from inspiring English sources

To understand the mechanism of hematopoietic failure in cas002 mutant, we carried out positional cloning.

To accurately investigate the possible more subtle differences between wt and S6′T mutant, we carried out three additional independent simulations for each system.

To check whether the regulatory role of Bcl2 in autophagy is responsible for the rescue of HSPC in kri1l cas002 mutant, we carried out immunoblotting on the whole embryo lysates using an antibody against an autophagy marker, microtubule-associated protein light chain 3 (Lc3).

To develop a rational design for high-activity mutants, we carried out free-energy perturbation (FEP) simulations on various mutations of the transition-state structures in addition to the corresponding free-enzyme structures by using an extended FEP procedure.

To evaluate the viability of homozygous D/D and heterozygous D/+ mutants, we carried out crosses between D/+ heterozygotes.

To determine the basis of the dorso-ventral patterning and pole cell defects in syp mutants, we carried out in situ hybridization and immunostaining for grk, osk and bcd mRNAs and Grk and Osk proteins.

As we could not identify a coding region mutation in Grhl2 in Axd mutants, we carried out additional sequencing of the 5′ and 3′ UTRs including 1.6kb upstream of the start codon (at chromosome 15: 37 163 142) and a conserved 5′ region (37 111 871–37 112 537).

To check whether the mutations contained in the site-directed mutants assayed can fix when present in a simple mutant spectrum, we carried out competition experiments of these viruses with the virus Qβwt.

To verify that the deletion in Os04g0101800 was responsible for the sor1 mutant phenotype, we carried out a complementation test.

In order to examine possible perturbations of the cell cycle caused by the genes disrupted in the mutant parasites, we carried out immunofluorescence assays (IFAs) with the cell cycle markers IMC1 and Centrin [27].

In order to test the utility of our mutant sets, we carried out additional pilot experiments using both in vitro and in vivo conditions.