To investigate the inhibitory effect of Phyllanthus extract on LMV-resistant mutants, we substituted methionine 204 with alanine, isoleucine, lysine, leucine, arginine, serine, threonine, or valine to generate YADD, YIDD, YKDD, YLDD, YRDD, YSDD, YTDD, or YVDD, respectively (Fig. 3a).
To generate this mutant α-syn, we substituted the lysine residues within α-syn with arginine, which is similar in size and charge to lysine.
We substituted JAK1 residue F636 to Ala in the context of V658F, and quantified the STAT3 activity of this mutant in the JAK1-deficient cell line, U4C.
Therefore, we substituted the lysine 49 with threonine (K49T) or alanine (K49A), and examined the binding of these Gab1-mutants with PTP1B-181A mutant.
To examine this possibility, we generated several PCAF mutants that substituted residues near the enzyme active site with an alanine.
(C) YnaIF209A mutant showed a higher conductance comparing with wide-type YnaI and YnaI mutant substituted with the TtMscS β-barrel region (YnaI-Ttβ) (n = 4, mean ± SE). (D) Left: single-channel traces of YnaI, YnaI-Ttβ and YnaIF209A mutant were recorded at +40 mV.
d1-1 was identified as spontaneous mutant 'Daikoku' and substituted its genetic background into Taichung 65 by backcrossing with Taichung 65 as a recurrent parent at Kyushu University.
N-terminally FLAG-tagged wild-type NS3/4A protease and its mutant substituted with Ser139 to replaced with Ala (S139A) were prepared as described previously [33].
In the central region, SPL and BEL1 coordinate to determine the identity of ovule chalaza where the integuments and nucellus are joined, since the chalaza of nzz bel1 double mutant was partially substituted by the tissue of the funiculus.
The obliteration of the capacitance response by substituting one of the Sx 1A mutants was substituted for Sx1A could result from difficulties in protein expression or targeting to the cell membrane, or both.
