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In order to determine whether OsUBC13 could functionally complement the error-free PRR defect in the yeast ubc13 null mutant, we performed yeast killing and spontaneous mutagenesis assays.
To investigate the cytological mechanisms responsible for the pollen abortion of oslap6 mutant, we performed transverse section analysis for the anthers of oslap6 and WT.
To determine whether this was the case in the msh4 mutant, we performed FISH of the elongated nucleus.
In order to monitor the expression levels for these clusters in the cuff mutant, we performed quantitative reverse transcription (qRT PCR) assays with a set of specific oligonucleotides.
To exclude the possibility that DNA hypomethylation might result from lower expression of the PHD and RING domain mutant, we performed a transient rescue assay in Uhrf1 −/− ESCs.
To provide further evidence that kri1l is defective in cas002 mutant, we performed a rescue experiment by microinjection of synthetic wild-type kri1l mRNA into cas002 mutant embryos.
Similar(45)
Therefore, to increase our likelihood of detecting any developed resistance mutants we performed our sequence analysis on DNA samples from the earliest possible time point at which HIV-1 DNA was detected.
For testing the stability of the different UNC-45 mutants, we performed limited proteolysis experiments.
To determine the virulence phenotypes of the PsAvr3c mutants, we performed an infection assay on Rps3c soybean seedlings.
In order to measure the copy number of rDNA repeat in Sup mutants, we performed pulsed field gel electrophoresis and subsequent Southern blotting using 5S rRNA gene as a probe (Fig. 3a).
To test whether tissues were undergoing apoptosis in ablated mutants, we performed whole-mount TUNEL staining.
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