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To identify the acetylated Hsp90 residues in the hda1Δrpd3Δ mutant, we turned to mass spectrometry.
Since functions of the motor complex provided no obvious mechanism for the transformation defect of the P clag - cdpk1-gfp mutant, we turned to quantitative mass spectrometry to ask whether the expression of additional proteins was altered.
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Given the lack of a motor behavioral phenotype in young adult Lrsam1 mutant mice, we turned to more detailed analyses in older mice, including an examination of neuromuscular junction (NMJ) morphology at 5 months and at >12 months of age to look for signs of die-back neuropathy (Fig. 4).
To validate the SEC observations and to confirm that the oligomerization state of the triple mutant was indeed concentration-dependent, we turned to a combination of analytical ultracentrifugation and SEC-MALS.
In order to overcome the weakness of the phenotype, we turned to GSK3 mutants as loss of this kinase can enhance aPKC levels and activity [8].
To compare transport defects in solely dysmyelinated optic nerves, we turned to the Plp1 mutant rumpshaker mouse (Schneider et al, 1992) that exhibits no demyelination.
Since delayed aging in the daf-2/insulin-like pathway mutants has been extensively documented, and likely involves hormonal control of aging in all tissues [1], [3], [15], [16], we turned our attention to mutants that more directly affect pharynx function.
We report here an expansion of these efforts by incorporation of an amino substituted variant of tryptophan into the "cyan" GFP mutant, which turned it into a "gold" variant.
But even that crew of mutant misfits turned protectors of humanity exists in a circumscribed imaginative space.
The only mutant that turned out to be susceptible to proteinase K digestion was V1G, which might be due to a change in the conformation of the protein in the N-terminal region.
A mutant female turned self-compatible hermaphrodite invaded the ancestral dioecious population because the probability of females failing to reproduce was higher than that of hermaphrodites in environments with limited outcrossing.
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