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For identifying the molecular lesion of the mutant, we filtered in Microsoft Excel the variant MAQGene output file for protein changing variants.
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WE filter this vast knowledge, my love!
We filtered out extreme outliers.
Next, we filtered p-values,.
We filtered all buffers (0.20 μm filter) prior to use.
To make sure that such cases do not interfere with the comparative evaluations, we filtered out any unambiguous interaction pairs, and for the remaining interactions, we used the same interaction class for the reciprocal mutant pairs.
We filtered marker pairs based on linkage.
From this database, which includes data for >3000 mutations, we filter only single-point mutations with available experimental affinities for both wild-type and mutant forms.
We filter out evidence that challenges them.
We filter such database entries.
Genes in Ypr013CΔ or Ypro015CΔ single mutant and double mutants (Ypr013CΔ Ypro015CΔ) were filtered based on flags present in four out of eight samples.
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Justyna Jupowicz-Kozak
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