Sentence examples for mutant we generated from inspiring English sources

In order to identify some potential stress and/or compensatory responses in the sgn3 mutant, we generated a general transcriptional profile of sgn3 mutant rosettes, grown under conditions in which wild type and sgn3 were indistinguishable.

To determine if either gene is involved in axon regeneration, we characterized regeneration in ttr-57 tm6877 -null ttr-57 tm6877 -null ttr-57 tm6877 -nullttr-11(kmutantutanimalsgenerated using the CRIPSR/Cas9 system (Fig. 1a) (see Methods).

To confirm the function of OsBEIIa in the sug-h mutant, we generated dsRNA-mediated interference (RNAi) transgenic plants.

To investigate LHP1 binding in the lif2 mutant, we generated a genomic LHP1 MYC construct; this genomic fragment had been previously used for lhp1 complementation [26].

In order to improve vaccine efficacy and safety of the ΔsecA2 mutant, we generated multiple double and triple mutants of the ΔsecA2 mutant strain via incorporation of other attenuating mutations of M. tuberculosis.

To test for dominant effects in the DFGx mutant, we generated heterozygous IME2/ime2-DFGx strains.

This describes the experiments for the monomorphic populations, where we retain the parental sequence if the single mutant we generate is nonfunctional.

To evaluate the expression and specific activity of mutants, we generated single-point mutations in the residues that differ from the sequence of the wild type in the mutants M1 to M6 using the PCR mutagenesis strategy (Kirsch and Joly 1998).

Finally, we utilized a series of CXCL10 mutants we generated to map the CXCR3 and GAG binding sites of CXCL10 [28].

To investigate whether the observed dendritic defects were to the result of affected deneddylation in CSN5 mutants, we generated MARCM clones of strong Nedd8 mutations.

In addition to insertion mutants, we generated three site-specific mutants based on prior studies of dnaQ.