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To characterize the yellow-green leaf phenotype of the ygl138 mutant, we measured its chlorophyll contents at the seedling stage.

To determine whether the silencing of endogenous meiosis-specific genes is defective in hip3-1 mutant, we measured SPCC663.14c+ transcript levels using real-time quantitative RT-PCR.

To assess whether photosynthetic electron transport is affected in the pgr7 mutant, we measured two key chlorophyll fluorescence parameters, the quantum yield of PSII (ΦPSII) and the reduction state of PSII (1-qL), as a function of light intensity (Figure 3).

To determine whether capn3a and capn3b were expressed normally in the def hi429 mutant, we measured their expression levels in zebrafish.

Because our previous experiments suggested slow DNA replication in the eco1 mutant, we measured the completeness of DNA replication genome-wide at late S phase.

In the aneuploidy (XY* model) mouse mutant, we measured Gh mRNA in mPOA and found a significant positive relationship between levels of Gh and numbers of X chromosomes [ 9].

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For a subset of the mutants we measured single-channel currents in planar bilayers.

To quantify observed differences in the repair activities of APE1 mutants, we measured steady-state kinetic parameters of the repair reactions and calculated the KM, kcat and kcat/KM values for WT APE1 and for four relevant mutants.

In order to quantify the efficiency of HPV16 infection in cells treated with dnEps15-mutants we measured the number of transfected cells expressing the DsRed-marker plasmid by FACS analysis.

To test whether insulin secretion was affected in the mutants, we measured serum insulin in response to a glucose challenge.

To examine whether neuron loss could explain SCN neuropeptide depletion in our mutants, we measured neuropeptide expression by ISH at P0, when Six3-Cre Lhx1lox/lox SCN neuron number is normal.

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