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Photoreceptor mutants were crossed with bas1-2 sob7-1, and multiple mutant combinations were isolated in F2 populations.
Specific multiple mutant combinations were reported to display sterility and a range of defects in meiotic chromosome behavior [ 83].
Instead, they have relied on the study of single or multiple mutant combinations to reveal the generation of phenotypic patterns (e.g. [ 1]).
The reason is that they are redundant with other MADS domain proteins so that phenotypic effects appear only in multiple mutant combinations [ 8].
ben1-1 was crossed with bas1-2 sob7-1, and multiple mutant combinations were isolated from populations of F2 individuals derived from self-pollinated F1 plants.
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To further examine genetic interactions between the BR-inactivating enzymes and photomorphogenic photoreceptors, we measured flowering time in multiple null mutant combinations of bas1-2 and sob7-1 with phyA-211, phyB-9, cry1-103, and phyA-211 phyB-9 grown in both long- (16-hr light:8-hr darkness) and short-day (8-hr light:16-hr darkness) conditions.
In the fully cooperative model, simultaneous acquisition of all five WT residues is required to confer a selective advantage, leading to a mean waiting time of 4.7 × 10 (arbitrary units) considering all 120 pathways of adding in the residues (black net rates; for simplicity, only one intermediate of the multiple possible mutant combinations is shown in each step).
It should also be mentioned that in each of the multiple FRI FLC mutant combination studies, fri-flc double mutant period was always reduced compared to FRI-FLC period.
We used homozygous and heteroallelic mutant combinations to assay behavior in multiple distinct genetic backgrounds.
In designing a screen of this type, there are trade offs between the advantages of mutating multiple codons and the disadvantages of needing to screen large numbers of transformants to recover all possible mutant combinations.
Simultaneous coevolution of multiple residues makes mutagenic studies of Rubisco functioning more laborious as with any new residue added the number of mutant combinations to be tested rises exponentially.
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several mutant combinations
various mutant combinations
multiple mutant molecules
multiple mutant transgenes
multiple mutant sites
multiple mutant experiments
multiple mutant genes
multiple rational combinations
multiple mutant genotypes
multiple mutant alleles
multiple mutant phenotypes
multiple mutant HγD-Crys
multiple mutant loci
multiple mutant backgrounds
multiple mutant tissues
multiple mutant data
multiple mutant haplotypes
multiple mutant versions
multiple mutant lines
multiple mutant strains
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