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Thirdly, the relatively low cost and large clutch size of zebrafish allow the ability to generate multiple mutant lines and provide statistical power for robust conclusions.
When there are multiple mutant lines in a family, a series of interconnected crosses between different mutant lines are necessary to resolve ambiguities.
We tested if hybridization of DNA from multiple mutant lines that exhibit a phenotype of interest could provide convergent data to identify the mutated region responsible for the phenotype and limit that region to the fewest gene models.
By analyzing multiple mutant lines, we report here that TCL1 functions redundantly with other single-repeat R3 MYB transcription factors to control both leaf trichome and root hair formation.
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We discovered that this allele has actually been inadvertently backcrossed into multiple mutant mouse lines, including two engineered to be deficient in Siae and Cmah.
In many cases, a gene had multiple insertional mutant lines, but the phenotypic effect was the same for all lines.
AS1 expression remained unchanged in the multiple yab mutant lines, with the exception of fil yab3 mutants, in which it showed a slight increase.
Second, multiple zebrafish mutant lines or knockdowns have been generated that recapitulate common human disorders, such as various types of anemia, protoporphyria, or thrombocytopenia [ 64, 70, 71].
We describe the isolation of multiple, independent mutant lines, including two (vt21 and vt22 ) that possess novel alleles of DAT-1.
Since multiple unrelated mutant lines of the same strain background were included in the analysis, the set of the remaining lines served as filter for private variants in each one of the lines.
In this study, resting on our characterization of multiple Smr mutant lines, mosaic analysis with a loss-of-function Smr allele, and knockdown experiments with two Smr.IR fly lines, we report the involvement of SMRTER in the development of ovarian follicle cells and wing.
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