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An alternate model, in which defective ciliogenesis underlies the observed abnormal outer segment development, is unlikely, based on the presence of normal-appearing cilia in multiple mutant tissues where Cc2d2a is normally expressed, including the kidney and retina, despite phenotypic anomalies in these organs.
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Among the multiple mutant phenotypes, pathogenicity-defective mutants were our primary focus in this study.
Histological analysis shows that Muc16 homozygous mutant tissues are normal.
Multiple mutant versions of Cas9, including Cas9D10A, Cas9H840A, and a Cas9D10A,H840A double mutant, were tested.
Photoreceptor mutants were crossed with bas1-2 sob7-1, and multiple mutant combinations were isolated in F2 populations.
The multiple mutants showed denaturation temperatures up to 27 deg.
There, the pollen grains from tir1 and afb1afb2afb3 multiple mutants age prematurely (Cecchetti et al. 2008).
Using sequence- and structure-based approaches, 48 CLF multiple mutants were engineered and analyzed.
However, combining newly developed mutant tissue-type plasminogen activators with coronary angioplasty should be reconsidered.
Double and triple mutants demonstrated some additive or synergistic effects, which were only inactivated by higher concentration H2O2, whereas multiple mutants A77E/A79S/I81L/M265L, A77E/A79S/I81L/M247L/M265L, and A77E/A79S/I81L/S168A did not.
This limited collection of mutant PAI-1 preparations contained multiple mutants defective in binding to three out of four tested anti-PAI-1 monoclonantibodiesies.
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