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For example, deleting the checkpoint sensor mec1 in multiple mutant backgrounds that extended S phase duration maintained the approximate scaling of the replication profile with S phase duration, and decreased rather than increased the number of active origins contributing to DNA replication.
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These models are validated through prediction of gene expression in mutant backgrounds.
We combined an smn heterozygous mutant background with either mutant backgrounds (where available) and/or with over-expression constructs of random genes out of our lists.
Among the multiple mutant phenotypes, pathogenicity-defective mutants were our primary focus in this study.
Comparison of variation among mth allele backgrounds supports allelism, as variation in the wild-type background was not greater than variation in the mutant backgrounds (Table 2).
Rps6 phosphorylation was reduced in all mutant backgrounds (Fig. 3A).
The previous experiments utilized mutant backgrounds for puc and hep.
Catalytic effects of the Mg2+ ion in different mutant backgrounds.
Furthermore, the delay seen in rng mutants is abolished in SIN mutant backgrounds.
Dachs protein levels do not change in Fbxl7 mutant, Fbxl7 overexpressing, or ft mutant backgrounds compared to control.
This is particularly useful when the genetic backgrounds of multiple mutants need to be maintained in a pure background.
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