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Of note is that in several cases our analysis identified multiple mutant loci, which then had to be individually assessed as candidate loci.
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While ciliary ultrastructure is normal in most mutants, one of the mutant loci is essential for the formation of proper microtubule architecture in the axoneme of pronephric cilia.
Genetic mapping of mutant loci was performed as described [18].
Among the multiple mutant phenotypes, pathogenicity-defective mutants were our primary focus in this study.
Mutant loci were identified by iPCR (Keim et al., 2004).
The multiple mutants showed denaturation temperatures up to 27 deg.
Slatkin (2008) presented a Bayesian method for jointly inferring selection intensity and allele age of the mutant using linkage disequilibria of multiple marker loci and generated the probability of data with an importance sampling algorithm.
Any significant deviation from this ratio could indicate that multiple loci contribute to the mutant phenotype (Appendix 1, Note 2).
Below, we propose a breeding scheme to harness multiple favourable loci.
Tags matching to multiple genomic loci were discarded.
If a single locus aligned to multiple map loci, we discarded all alignments for that locus.
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