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Mouse studies using the GFN have disentangled the effects of macronutrients on fatty liver and the gut microbiome.
Relevantly, the dependence of some spatial learning paradigms on adult hippocampal neurogenesis has been revealed in two mouse studies using genetic ablation of neurogenesis but not in others [53].
The pathogenic role of B cells in SLE has been highlighted by mouse studies using either B-cell-deficient mice or B-cell depletion [ 25, 26].
In vitro studies using mammary fibroblasts [ 29] and in vivo mouse studies using CAFs and NMFs [ 70] indicate that the effect of fibroblasts on tumour cells depends upon the type and proportion of inoculated cells.
Using a translational approach we conducted a patient study in patients with culture-confirmed sepsis caused by B. pseudomallei, in vitro experiments using wild-type (WT) and uPAR knockout (KO) cells, and mouse studies using WT and uPAR KO mice inoculated with B. pseudomallei.
The dietary positive control (0.5 ppm E2) was selected based on one-generation (Tyl et al. 2008a) and two-generation (Tyl et al. 2008b) CD-1 mouse studies using E2 doses of 0.001 50 ppm (8 mg/kg/day), with the most complete spectrum of effects seen at 0.5 ppm [estrogenic effects were observed at 0.05 ppm (increased weanling uterine weight) with a NOAEL of ~ 1 μg/kg/day].
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The majority of mouse studies use male C57Bl/6J mice as a model for obesity induced by a high-fat diet (HFD) and assess only gWAT, whereas sWAT and mWAT are neglected [ 17].
Although our mouse study using E11.5 Shh mutant embryos shows an early differentiation defect, such results in human (E11.5 mice correspond to a 10-week-old fetus) are only speculative because such samples are rare and difficult to obtain.
In vivo mice studies using orthotopic xenograft mouse model also confirmed these results.
The mouse study used GeneChip Mouse Genome 430 2.0 arrays (Affymetrix, Santa Clara, CA), which contain over 45,000 probe sets that target transcripts that represent over 34,000 mouse genes.
Our phenotype data was obtained from previously published mouse phenotyping studies using transgenic or knockout mice.
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