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Wolff, A. et al. Distribution of glutamylated alpha and beta-tubulin in mouse tissues using a specific monoclonal antibody, GT335.
We analyzed the distribution of m-spinesin variants in mouse tissues, using RT-PCR with variant-specific primer sets.
Next, we evaluated the expression pattern of the Noc4l gene in adult mouse tissues using RT-qPCR.
Total RNA was isolated from cell lines or mouse tissues using the RNeasy kit (Qiagen).
Total RNA were isolated from mouse tissues using the Trizol method (invitrogen).
Total RNA was extracted from mouse tissues using Trizol reagent (Invitrogen) according to the manufacturer's instructions.
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Total RNA was extracted from mouse tissue using TRI-Reagent® (SIGMA-Aldrich, Taufkirchen, Germany).
Total RNA was extracted from cultured cells or mouse tissue using TRIzol (Invitrogen).
To analyze the effects of targeting these receptors systemically in vivo, we silenced ADMR on both human cancer cells and on mouse tissue using in vivo delivery of siRNA in DOPC nanoliposomes.
Mitochondria were isolated from mouse tissue using differential centrifugation as previously described (31).
miRNA was isolated from mouse tissue using the miRNeasy Mini Kit (Qiagen), following the manufacturer's instructions.
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