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Appropriate results were previously obtained for these control mouse samples using Western blot and ECLISA.
FISH was used to detect M. tuberculosis in infected mouse samples using fluorescent M. tuberculosis probes MTB187, MTB223 and MTB1284.
Detailed analysis of the rat and mouse samples using this protocol was published in a separate paper.
Several recent studies have compared transcript expression levels measured in human and mouse samples using both conventional microarrays and RNA-Seq [ 13, 16].
The gene expression level was normalized relative to GAPDH expression level for chicken samples and relative to HPRT expression level for mouse samples using a ΔCt approach.
For example, Lee et al. [ 109] conducted a comprehensive survey of miRNA sequence variations from human and mouse samples using RNA-Seq.
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This suggests that, while the mouse samples used in this study may have displayed morphological features of blastocyst formation, the ICM cells had not yet undergone lineage specification to EPI or PE.
We determined HbA1c from mouse blood samples using the A1CNow + kit from PTS Diagnostics (Indianapolis, IN).
For PCR genotyping, DNA was extracted from mouse tail samples using a DirectPCR Lysis Reagent (Viagen Biotech Inc., Los Angeles, CA) following the manufacturer's guidelines.
In order to investigate the possibility of microbial symbiotic origin of DING proteins in eukaryotes, we analyzed wild-type and germ-free mouse plasma samples using western blot assays.
DNA was extracted from mouse tail samples using DNeasy ™ Blood and Tissue Kit (Qiagen).
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