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Here, we determine the respective contribution of lipids and proteins to MRI contrast by removing lipids and preserving proteins in mouse brains using CLARITY.
"The first thing we asked when we saw that was, what happens if you bring gamma back?" says Tsai. Tsai's lab had already induced gamma waves in mouse brains using optogenetics back in 2009, so the researchers knew how to do it.
A noncontact electric field was applied at a specific local blood vessel to detect effective cerebral blood volume increases in mouse brains using in vivo optical recordings of signal imaging.
Total RNA was extracted from fresh 4-month-old mouse brains using Trizol (Invitrogen) standard extraction.
Total RNA, including small RNAs, was extracted from fresh 4-month-old mouse brains using the mirVana miRNA Isolation Kit (Ambion).
We fractionated mouse brains using an iodixanol gradient fractionation system identical to that described in the published cell culture study [4] (Fig. 5).
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Purified total RNA from the infected and uninfected mouse brains used for RNA-seq was also used as templates for cDNA synthesis for qRT-PCR assay.
Saito, T. & Nakatsuji, N. Efficient gene transfer into the embryonic mouse brain using in vivo electroporation.
First you'll witness a "fly-through" of a complete mouse brain using fluorescent imaging.
Tabata, H. & Nakajima, K. Efficient in utero gene transfer system to the developing mouse brain using electroporation: visualization of neuronal migration in the developing cortex.
This study was designed to track systemically administered mononuclear cells (MNCs) in the ischemic mouse brain using 7 T magnetic resonance imaging (MRI).
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