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Total RNA was extracted from mouse livers using Trizol Reagent (Invitrogen) and further purified using RNeasy Mini Kits (Qiagen).
Total RNA was prepared from cell cultures and mouse livers using RNABee™ (Biozol).
We reconstructed the miRNA-mediated regulatory network in mouse livers using high-throughput expression profiles.
Total RNA was extracted from both rat and mouse livers using Qiagen RNeasy kits according to the manufacturer's instructions (Qiagen, Mississauga, Canada).
Total RNA was isolated from frozen mouse livers using Trizol Reagent (Invitrogen Corporation, Carlsbad, CA) and further purified using the RNeasy Mini Kit (Qiagen Inc., Valencia, CA).
Methionine was measured in extracts from Wt and Nox4-/ mouse livers using a Bridge-It® l-Methionine (l-Met) Fluorescence Assay (mediomics) as per the manufacturer's protocol.
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Briefly, total RNA was extracted from 200 ml of human serum or 100 mg mouse liver using the Trizol Reagent (Invitrogen) according to the manufacturer's instructions.
To test the applicability of our method for archival tissue, we extracted 11-month old FFPE mouse liver using the pressure-assisted protocol developed for our model systems.
This methodology was demonstrated by performing ChIP on hepatocytes isolated directly from mouse liver using well-characterized antisera against the liver-enriched transcription factor Hnf4α [7], [15, [15] and the trimethylated lysine 4 of histone H3 [3], [5], [12] [12].
The mMCSF cDNA fragment was cloned from mouse liver using primers for the cloning into the NdeI-XhoI sites of the expression vector pACYCDuet-1 (Novagen), and the resulting plasmid was designated pSS110.
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