Sentence examples for min before lysis from inspiring English sources

100 µg/ml of cycloheximide was added to the cells for 1 min before lysis.

Where indicated the cells were exposed to thapsigargin (Tg, 1 µM) for 5 min before lysis.

In one experiment involving pY477 ezrin western blotting, cells were treated with 0.1 mM pervanadate for 5 min before lysis to inhibit phosphatases [ 6].

For some strains, the drop in turbidity was immediate, while with others there was a lag of up to c. 20 min before lysis was detected.

In those studies, cells were stimulated with tunicamycin for 15 min before lysis, thus demonstrating a reduced ability to phosphorylate eIF2α in the presence of ER stress.

The suspension was incubated on ice with 1 mg·mlysozymezyme for 30 min before lysis by sonication (VibraCell, Jencons PLS), clarified by centrifugation and purified by Ni-chromatography on an ÄKTA Purifier system (GE Healthcare) at 4 °C.

To measure DNA repair capacity, we incubated a second batch of BLEO-treated cells for 15 min (37°C) before lysis and electrophoresis.

To examine adhesion properties of bacterial strains, the infection time was reduced to 35 min, and before lysis, cells were washed four times with PBS/Mg2+Ca2+.

However, our observation that pyroptotic cells undergo cell volume increase up to 10 min before total lysis (Fig. 6) suggested that pyroptotic cells have permeability of their plasma membrane prior to total lysis, as marked by Sytox Green.

We serum starved the cells for 24 h and added IGF1 either during the whole starvation period or during the last 10 min before cell lysis (Figure 5C).

C2C12 cells transfected with pcDNA3.1-JunB plasmid or treated with BMP2 were fixed by 1% formaldehyde for 10 min before cell lysis.