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In contrast, progesterone 30 min before restraint offered no protection.
In the first experiment, ovariectomized rats, primed with 10 μg estradiol benzoate, received 500 μg progesterone 4 h, 1 h, or 30 min before restraint.
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This is compared with the second group of previously unstressed animals (controls) restrained for 240 min on day 5. Surgery is carried out 4 h after the fourth daily restraint and 24 h before restraint on day 5. Statistical analysis are always carried out on raw data (concentrations: pg/20 μl): these are presented in figures as percent changes from baseline levels (Fig. 5).
The next week, females were primed with 10 μg estradiol benzoate two days before a 5 min restraint.
When progesterone was injected 4 h before restraint, progesterone eliminated the effects of restraint.
We have reported that 5 min of restraint reduced lordosis behavior of ovariectomized rats hormonally primed with estradiol benzoate.
The second occurred at the same time of day but followed 20 min of restraint stress in a Plexiglas tube.
While circulating ACTH levels increased after 5 min of restraint, levels were not greater in chronic stress animals compared to controls until following 20 min.
Before restraint, lordosis behavior was comparable across treatment conditions but only progesterone-treated rats exhibited proceptive behavior.
Stressed samples were obtained from separate individuals (n = 5) after 60 min (63.43±0.59 min) of restraint in an opaque cloth bag.
In zebra finches, plasma CBG levels decrease after 60 min of restraint [15], which might affect the compartmentalization of corticosterone between plasma and RBC.
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min before administration
min before measurement
min before collection
min before treatment
min before infection
min before conditioning
min before stimulation
min before analysis
min before mealtime
min before reperfusion
min before observation
min before transfer
min before ischemia
min before irradiation
min before resuspension
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