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The mixture was incubated on ice for approximately 30 min before plaque titering on Vero cells seeded on 12-well plates as duplicates.
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In this line, cognitive deficits are evident before plaque formation.
APP23 mice display cognitive impairments before plaque formation, while deltaE9 mice develop abundant plaques before the decline of cognitive performance.
Imaging studies provide further support for this hypothesis with observations of microglia activation before plaque formation.
These mice displayed early synaptic dysfunction before plaque or tangle deposition was evident, together with early intraneuronal Aβ immunoreactivity preceding plaque deposition.
Heparin indeed reduced the formation of plaques by 50% when added 30 min before infection of Vero cells with 100 PFU/mL of the SARS-CoV.
140 min before 8-OH-DPAT injection (time 0 min).
Cooling began 30 min before reperfusion.
were administered 30 min before HCl instillation.
pretreatment 30 min before morphine injection.
Baseline samples were collected 15 min before CCI (under 1 2% isoflurane), 5 min pre-CCI, 10 min post-CCI, and 60 min post-CCI.
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