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Saline or nicotine was administered 5 min before training and/or extinction.

In accordance with procedures described elsewhere [20], each animal (n = 9 per group) was placed in darkness 5 min before training.

Male and female CD1 mice were administered i.p. 30 min before training, as follows.

We found that blockade of BDNF with function-blocking anti-BDNF antibodies delivered into dorsal CA1 15 min before training abolished the IA learning-induced increase in mTOR (Figure 5A) and p70S6K (Figure 5B) phosphorylation.

To determine whether the BDNF/mTOR pathway regulates these increases in GluR1 protein in synaptic plasma membranes, we injected into CA1 region of the dorsal hippocampus function-blocking anti-BDNF antibodies or rapamycin 15 min before training and sacrificed the animals 15 min after training.

To analyze the effect of mTOR and BDNF inhibition on GluR1 expression levels, IA-trained receivedeived bilateral intra-CA1 infusions of rapamycin (4.3 pg/side), anti-BDNF (0.5 ug/side) or saline 15 min before training and were killed by decapitation 15 min thereafter.

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Similar(53)

Using a 2×2 design, rats were infused with either bacteriostatic water or 30 μl/1 μl of scopolamine HCl into the frontal cortex 30 min before being trained on passive avoidance.

Mice (n = 9/experimental group) were acclimatized in a 40×40 cm square cage made of white coated plywood with 16 cm-high walls for 15 min per day during the week before training.

For example, in the study by Morgan et al [ 9] VE averaged 160 L/min during maximal exercise before training and 175 L/min after training, while McMahon et al [ 5] reported that sub maximal exercise VE rose from 147 L/min before training to 156 L/min after training.

Have a plan before training.

Always warm up before training.

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