Sentence examples for min before analyses from inspiring English sources

After two washing steps in cold PBS, RNAse A and PI were added to a final concentration of 50 mg/ml each and incubated at 4°C for 60 min before analyses.

Prior to cell cycle analysis, cells were resuspended in PBS containing RNase A (20 ug/mL, Sigma) and 20 µg/ml propidium iodide (PI; Sigma), and then incubated at 37°C for 30 min before analyses for PI fluorescence intensity using the Becton Dickinson FACsort flow cytometer (Becton-Dickinson, Temse, Belgium).

Reaction mixtures were centrifuged (1400 r.p.m., 10 min) before analyses of supernatants.

The serum samples were inactivated at 56°C for 30 min before analyses.

Cells were subsequently incubated in the dark (22°C, 30 mins) before analysing using a Tali Image-Based Cytometer (Invitrogen, USA).

We then added 50 μg/mL propidium iodine 5 min before FACS analyses.

The tube was kept in a 35°C water bath (Grants Instruments Ltd., Cambridge, UK) for 5 min before semen analyses.

Blebbistatin (Sigma Aldrich, St . Louis MO) was used at 50 µM and applied directly to the cells for 5 min before AFM analyses.

For the analyses of cell cycle, the treated cells were fixed in 70% ethanol and stored at −20°C overnight; the cells were labeled with propidium iodide (50 μg/ml) and RNase (100 μg/ml) for 30 min before the analyses by flow cytometry with Multi-cycle system software package.

A total of 0.2 × 10 cells were fixed overnight in 70% ethanol, washed twice in PBS and resuspended in 300 µl PBS with 10 µg/ml DAPI and 200 µg/ml RNase A. Cells were incubated at 37°C for 30 min before being analysed on the flow cytometer.

Briefly, 0·2 × 10 cells were washed twice in PBS and resuspended in 300 μl PBS with 10 μg/ml Hoescht 33342 and 0·5 μg/ml Pyronin Y. Cells were incubated at 37°C for 45 min before being analysed on the flow cytometer.