Sentence examples for min before analyzing from inspiring English sources

The thermal stability of YD1 samples was determined by exposure to 20, 40, 60, 80 and 100 °C for 30 min and to 121 °C/105 kPa for 15 min before analyzing the residual activity.

Counterstaining with 5 μg propidium iodide/mL cell suspension was done 1 min before analyzing.

Freshly prepared Calpain-Glo™ Reagent was mixed with samples (1 1) and added to each well of a white 96-well plate containing 40 μl of blank or test sample and incubated at room temperature for 15 min before analyzing on a LumiCount luminometer.

Samples were treated with RNase A (100 μg/ml) for 10 min at 37°C before analyzing by SDS-PAGE autoradiographyautoradiography

We sequenced the amplified regions in the Col-0 and Ws-2 parents and the F2 individuals with the predicted CO in sequencing by assembling reactions containing 1 µM primer, 0.5 µL of BigDye Terminator ready reaction mix, and 1X sequencing buffer and incubating for 20s at 96°, followed by 29 cycles of 10 sec at 50° and 4 min at 60° before analyzing on an ABI 3730xL sequencer.

The positive control was heat treated at 80 °C for 10 min and cooled on ice for 5 min before being analyzed a second time.

The cells were harvested and washed twice, then resuspended in Indo 1/AM (3 μg/ml) and incubated at 37°C for 30 min before being analyzed by flow cytometry.

The lysates were mixed with an equal volume of 2x loading dye (Sigma-Aldrich) and incubated at 37°C for 10 min before being analyzed by SDS-PAGE.

The sample was then incubated in the dark at room temperature for 30 min before being analyzed with a BD FACScan™ flow cytometer (BD Biosciences, San Jose, CA) using CellQuest Software (BD Biosciences, San Jose, CA).

The cells were stained with 5 μL Alexa Fluor 647 Annexin V and 7-AAD Viability Staining Solution and incubated in the dark at room temperature for 15 min before being analyzed by multicolor flow cytometry.

The cells were harvested, washed twice, re-suspended in 500 μL of DiOC6 (4 μM) and incubated at 37 °C for 30 min before being analyzed by flow cytometry to detect the changes in ΔΨm.