Sentence examples for min before analysis from inspiring English sources

The sample catalyst was placed in ethanol in an ultrasonic bath for 30 min before analysis.

A drop of the suspension was then placed on a copper grid and left to dry for 20 min before analysis.

The samples were ashed, dissolved in 20% nitric acid, and boiled on a hot plate for 15 min before analysis by inductively coupled plasma optical emission spectrometry with a model Optima 4300 DV (PerkinElmer, Norwalk, CT, USA).

CB[7]4 was incubated with RAW264.7 cells for 20 min before analysis.

The dye (10  μg ml−1) was added to cell culture 30 min before analysis.

The reactions were performed at 30°C for 30 min before analysis on SDS-PAGE.

Cells were subsequently stained with optimally diluted antibodies against surface markers on ice for 60 mins, followed by fixation with 4% paraformaldehyde in PBS at room temperature for 20 mins before analysis by laser scanning confocal microscopy (Nikon).

The CB[7]5 complex was incubated with cells for 20 min. at a concentration of 32 µM the following day and then chased with infection medium for 15, 45, and 120 min. Before analysis, cells were fixed with 4% PFA, washed and immobilized with Prolong® Gold Antifade Agent (Invitrogen).

In our current PDMS-needle biosensor system this requires >1 m of connection tubing between the probe and the PDMS chip, leading to a 25 min delay before analysis at 1 μL/min flow rate, as well as a broadened temporal response due to Taylor dispersion.

After harvesting, both floating and adherent cells were collected and resuspended in 0.5 ml of PBS and LDS-751 (Exciton, Dayton, OH, USA) to a final concentration of 100 nM and incubated at room temperature for 20 min, and before analysis 10  μl of TO-PRO-3 iodide (1 nM; Molecular Probes, Eugene, OR, USA) were added.

The culture broth before and after replacing medium was centrifuged (1600×g, 4 °C, 15 min) before TLC analysis.