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For the AC treatment, CIDR were not soaked in disinfectant but were steam sterilized at 121 °C and 724 mmHg for 20 min before use.
The test suspensions were sonicated for 20 min before use.
Silica particles were sonicated for 5 min and vortexed for 1 min before use.
Firstly, the collected BoNT-negative sera were kept in room temperature for 10 min before use.
The gold-coated substrate was then annealed in H2 flame for 1 min before use.
The agar media were air-dried for 30 45 min before use.
Gold-coated or bare gold substrates were annealed in H2frame for 1 min before use.
The cultural medium was sterilized at 121 °C for 20 min before use.
The GO dispersion was homogenized by applying an ultrasonic treatment for 5 min before use.
The NPs were sterilized with UV irradiation for 30 min before use.
The reactor, hydration flask and all tubing were autoclaved at 121 °C for 20 min before use.
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