Sentence examples for microarrays were prehybridized from inspiring English sources

Before hybridization of the microarrays, 600 mJ of UV energy was applied to the Corning microarrays using a UV-chamber (Bio-Rad, Richmond, CA, USA) and the microarrays were prehybridized for 45 60 minutes at 42°C in pre-warmed prehybridization solution (5× SSC, 0.1% SDS and 0.1 mgml-1 BSA).

Briefly, the microarrays were prehybridized, the Cy5- and Cy3-labeled cDNAs were mixed with hybridization buffer (30 µl final volume), applied to the microarray, and covered with a Hybri-slip HS (Sigma-Aldrich, Taufkirchen, Germany).

The microarrays were prehybridized immediately preceding hybridization as described on the PFGRC website.

The microarrays were prehybridized at 50°C for 2 h and hybridized at 42°C for 14 16 h.

The cDNA and long-oligonucleotide microarrays were prehybridized in 40 μl of 5XSSC with 0.1% SDS and 1% BSA at 42°C for 30 minutes.

Microarrays were prehybridized in 1% bovine serum albumin, 5× SSC, 0.1% SDS for 45 min at 42°C, washed in H2O and dried by centrifugation.

All microarrays slides were prehybridized using 100 μL of DIG easy buffer (Roche Applied Science, Meylan, France) in humidified chambers for 1 hr at 42°C.

The microarray slides were prehybridized before use for 30 60 minutes in a 5×SSC/0.1 % SDS/0.1 % BSA solution at 42°C, according to the UltraGAPS™ Coated Slides instruction manual from Corning.

Before the hybridization, microarray slides were prehybridized in 5x SSC, 0.05% SDS, 4% BSA solution for 30 min at 45°C, washed twice with RNAse-free water for 2 min, rinsed with isopropanol and spin-dried for 3 min at 1500 g.

The microarray slides were prehybridized by incubation in 5× SSC, 0.1% SDS, 1% BSA at 42°C.

The microarray slides were prehybridized by incubation in 5X SSC, 0.1% SDS, 1% BSA at 42°C for 45 to 60 min. For each slide, the labeled cDNA probe was brought to 30.5 μl with the addition of sterile water.