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Exact(19)
Finally, the as-collected 2D VO2 microarrays were dried at 200 °C for 2 h in vacuum.
Microarrays were dried using a bath of acetonitrile (VWR, Fontenay sous Bois, France).
Finally, the microarrays were dried in a centrifuge at 2000 rpm for 3 minutes.
The microarrays were dried immediately by centrifugation and scanned using an Axon GenePix 4000B scanner (Molecular Devices).
Following the last wash the microarrays were dried by centrifugation in a low-ozone environment (<5 ppb) to prevent destruction of Cy dyes [72].
After a stringent wash for 10 minutes at 95°C in 1% SDS, microarrays were dried by centrifugation and scanned at 5 µm resolution on a GenePix 4000B scanner (Molecular Devices, Sunnyvale, CA) and analyzed using software developed in-house (see below).
Similar(41)
The microarray was dried in an ozone-free centrifuge by spinning at 300rpm for 4 minutes at room temperature and scanned using either Axonscanner 4000A or 4000B (Molecular Devices).
The rinsed microarray was dried by centrifuging at 500rcf for 4 min. The hybridized microarrays were scanned by GenePix scanner 4200 (Molecular Devices, CA) to obtain raw data sets.
Microarray slides were dried and dual channel images were captured using GenePix 4000B microarray scanner (Axon Instruments, CA).
Following hybridization, the slides were washed twice in 2× SSC, 0.1% sodium dodecyl sulfate at 42°C for 10 min, followed by twice in 1× SSC at room temperature for 10 min, and finally twice in 0.2× SSC at room temperature for 5 min. The microarray slides were dried by centrifugation at 300×g for 10 min before scanning.
Microarray slides were dried by centrifugation for 5 min at 300 rpm by using an Eppendorf 5804-R tabletop centrifuge.
Related(20)
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