Sentence examples for microarrays were incubated from inspiring English sources

First, the protein microarrays were incubated with a PBS blocking solution containing 1% BSA and 0.1% Tween20 for 1 hour at 23 °C to reduce non-specific binding.

Microarrays were incubated overnight (16 20 hours) at 50°C in a dark humidified chamber (Genetix).

For labeling, microarrays were incubated for 30 min with ExtrAvidin Peroxidase (Sigma) diluted 1∶1000 in BSA Peroxidase Stabilizer (BioFX).

Afterwards, the microarrays were incubated for 16 h in a wet chamber to ensure efficient covalent binding of the oligonucleotides to the surface.

Initially, microarrays were incubated for 30 min at 45°C in 50 µl of a solution consisting of 5 ml of 2×hybridization solution (see below), 1 ml of 50×Denhardt's solution (Sigma) and 0.5 ml of 1% SDS (Sigma).

Microarrays were incubated overnight at 37°C.

Each one of four microarrays was incubated with 30 µl of one of four different extension solutions (A, C, G or U) containing 1 x extension buffer (26 mM Tris-HCL pH 9.5, 6.5 mM MgCl2), 3.2 µM array-specific Cyanine-labeled dideoxynucleotides (ddNTP) and 6.6 U/µl thermosequenase DNA polymerase (USB Corporation).

During approximately 16 h, in which microarrays are incubated in a hybridization oven set to 45 °C, the cRNA binds to the specific probes attached to the glass surface of the microarray chip.

14 As a screen to identify materials with broad spectrum resistance to bacterial attachment, the polymer microarray were incubated with three different green fluorescent protein (GFP -labelled bacterial species, Pseudomonas aeruGFP -labelledStaphylococcus aureus 8325-4 and uropathogenic Escherichia coli O6:K15:H31 (UPEC) for 72 h.

To visualise antibody binding, the microarray was incubated with 3′-diaminobenzide and counterstained with Mayer's Haematoxylin.

The microarray was incubated for 90 minutes at RT in the dark.