Within each dataset, any genes not covered by our microarray were excluded from analysis.
The two genes (AQP and HBB) for which the RT-PCR was not consistent with the microarray were excluded from further analysis.
However, any derepression of retrotransposon expression in Tex19.1 −/− placentas would not be detected in the gene expression microarray analysis as any retrotransposon probes in the microarray were excluded from the gene expression analysis.
Each array included in the final analysis had at least 30% of the probesets detected above background (percent present >30%) and a 3' to 5' ratio of signal intensity for GAPDH of less than or equal to 5. One never smoker microarray was excluded based on these quality control filters (low percent present, high 3' to 5' GAPDH ratio), leaving four never and four current smoker arrays for analysis.
One such gene is EB129884 an α-expansin homologue, identified as highly expressed in 21 DAA fruit in the Fuji microarray, was excluded from the Royal Gala microarray by ANOVA analysis because two samples (132 and 146 DAA) had no detectable expression.
Anonymous ESTs from the microarrays platforms were excluded due to the inability to cross validate the identities between different gene expression profiles.
The 63 cases with technical failure in microarray mapping were excluded from the study.
For statistical reasons, genes that were not present in the entire microarray analyses were excluded from the related comparisons [ 17].
All hybridization images were inspected for artifacts, and aberrant spots or microarray regions were excluded from analyses.
The microarray probe to the haemagglutinin gene cntC (CBO0803; previously ha34) failed to give a satisfactory signal and these microarray data were excluded from the analysis.
