The microarrays were exposed to phosphorimager screens for 3 days.
Microarrays were exposed on a radioimager during 48 h and scanned using a Fuji BAS5000 machine (Fuji, France).
Microarrays were exposed for 1 3 d and scanned using a PhosphorImager (Molecular Dynamics, Sunnyvale, CA) at a 50-μm pixel resolution.
Reproduced in Figure 2A is the fluorescence image where the microarray was exposed to 10 PFU of H1N1 Influenza virus A/Brisbane/59/2007.
Thus, the signal intensity ratio used for gene expression calculations changes with the amount of time the microarray is exposed to ozone.
The microarray was exposed to three different strains of influenza virus, two H1N1, A/Brisbane/59/2007 and A/Solomon Islands/3/2006 and a H3N2, A/Aichi/2/1968.
Microarrays (from A to H) were exposed to a phospho-imager screen for eight days and scanned with a phospho-fluoroimager (Storm 860, Molecular Dynamics®).
Microarrays (from S0 to S5) were exposed to a phospho-imager screen for eleven days and scanned with a phospho-fluoroimager (Storm 860, Molecular Dynamics®).
For the microarray analysis, E. crypticus adults were exposed to 145 mg Zn/kg soil (EC50) and control soil for 4 days using four replicates, containing 5 7 individuals per replicate.
The microarray showed that after A549 cells were exposed for 1 hour to MFs, 19 cell cycle- and apoptosis-related genes had 2-fold upregulation, especially TNFRSF21 and CASPASE, and 40 genes, including ATM, p53, p57, p21, p27, TNFSF12, TNFRSF10D, BAG4, BCL2L2, Mdn2, and XRCC1 5, had 2-fold downregulation.
To carry out the microarray experiments, HUVEC from 9 separate cultures were exposed to control (0.1% ethanol) and 1 nmol/L estradiol treatments for 24 h.
